rabbit anti-human cd8 polyclonal antibodies  (Santa Cruz Biotechnology)

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Concentrations of primary antibodies.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques: Concentration Assay

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: (A) HE(x200). (B) Immunohistochemical (IHC, x400) CD8+, CD4+, FOXP3+Tregs, and PD-L1 of Tonsil (positive tissue) and breast tumor. (C) Immunofluorescence (IF, x20) Blue: DAPI; Red: CD105; Green: NG2 (The short arrow shows microvessels not covered by pericyte cells, the long arrow shows microvessels covered by pericyte cells).

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques: Immunohistochemical staining, Immunofluorescence

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Changes of sTILs, PD-L1, MVD, and MPI in non-pCR group and pCR group after NAC.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Univariate and multivariate analysis of pCR after NAC.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: Relationship between the baseline ratios of populations and pCR.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: The relationship between the changes of sTILs, PD-L1, MVD, MPI, and DFS before and after NAC. (A) Survival analysis between pCR group and Non-pCR group. (B) Survival analysis of sTILs change. (C) Survival analysis of CD8+T change. (D) Survival analysis of CD4+T change. (E) Survival analysis of FOXP3+ Tregs change. (F) Survival analysis of PD-L1 change. (G) Survival analysis of MVD change. (H) Survival analysis of MPI change.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Frontiers in Oncology

Article Title: Changes in Tumor-Infiltrating Lymphocytes and Vascular Normalization in Breast Cancer Patients After Neoadjuvant Chemotherapy and Their Correlations With DFS

doi: 10.3389/fonc.2019.01545

Figure Lengend Snippet: The relationship between DFS and sTILs, PD-L1, MVD, and MPI in Metastatic lymph node (LN). (A) Survival analysis of LN sTILs. (B) Survival analysis of LN CD8+ T cells. (C) Survival analysis of LN CD4+ T cells. (D) Survival analysis of LN FOXP3+ Tregs. (E) Survival analysis of LN PD-L1. (F) Survival analysis of LN MVD. (G) Survival analysis of LN MPI.

Article Snippet: CD8 rabbit anti-human polyclonal antibody (17335-1-AP) , Proteintech, US , 1:1,000.

Techniques:

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Association between the numbers of CD4- and CD8-positive lymphocytes in hepatocellular carcinoma tumor parenchymal tissues, and patient clinicopathological characteristics.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Biomarker Discovery

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Association between the numbers of CD4- and CD8-positive lymphocytes in hepatocellular carcinoma tumor stromal tissues, and patient clinicopathological characteristics.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Biomarker Discovery

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Immunohistochemical analysis of T-cell expression in the TP and TS of hepatocellular carcinoma samples (original magnification, ×400). (A) CD4 + and (B) CD8 + T-cell expression in TP and TS. (C) Morphometrical analysis was performed. Data are presented as the mean ± standard error of the mean. ** P<0.01, TP vs. TS in CD4 and CD8; †† P<0.01, CD4 vs. CD8 in TP; † P<0.05, CD4 vs. CD8 in TS. Statistical analyses were performed using the Mann-Whitney U test. TP, tumor parenchyma; TS, tumor stroma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Immunohistochemical staining, Expressing, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Immunohistochemical analysis of CD8 + T-cell expression in the TP and TS of hepatocellular carcinoma samples (original magnification, ×400). CD8 + T-cell expression in patients with tumor diameters (A) ≤5 cm and (B) >5 cm. (C) Morphometrical analysis was performed. Data are presented as the mean ± standard error of the mean. * P<0.05, diameter ≤5 cm group vs. diameter >5 cm group in TP and TS. TP, tumor parenchyma; TS, tumor stroma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Immunohistochemical staining, Expressing

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: CD8 expression in hepatocellular carcinoma (TP) and paired peritumor liver tissues (PTP). Morphometrical analysis was performed for semi-quantitative evaluation of the immunohistochemical findings. Data are presented as the mean ± standard error of the mean. Statistical analysis was performed using the nonparametric Mann-Whitney U test. ** P<0.01, PTP vs. TP in CH and C background groups. NCH, non-chronic hepatitis; CH, chronic hepatitis; PC, pre-cirrhotic stage; C, cirrhosis; PTP, peritumor parenchyma; TP, tumor parenchyma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Expressing, Immunohistochemical staining, MANN-WHITNEY

Journal: Oncology Letters

Article Title: Clinicopathological analysis of CD8-positive lymphocytes in the tumor parenchyma and stroma of hepatocellular carcinoma

doi: 10.3892/ol.2014.2516

Figure Lengend Snippet: Correlation between CD4 and CD8 expression in (A) TP and (B) TS of HCC samples. Statistical analyses were performed using Spearman’s correlation coefficient by rank test. P<0.001. TP, tumor parenchyma; TS, tumor stroma; HCC, hepatocellular carcinoma; h.p.f., high-power field.

Article Snippet: The sections were then incubated for 15 min with rabbit anti-human CD4 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc., CA, USA) and rabbit anti-human CD8 polyclonal antibodies (1:100; Santa Cruz Biotechnology, Inc.) in phosphate-buffered saline containing 1% normal goat serum (Wako Pure Chemical Industries, Ltd., Tokyo, Japan) and 1% bovine serum albumin (Wako Pure Chemical Industries, Ltd.) under intermittent microwave irradiation, as previously described ( , ).

Techniques: Expressing

Journal: The Journal of Experimental Medicine

Article Title: Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1

doi: 10.1084/jem.20082173

Figure Lengend Snippet: Tumor-derived monocytes induced T cell suppression via PD-L1. (A) Physical contact between PD-L1 + cells (red) and CD8 + cytotoxic T cells (green) in HCC peritumoral stroma. One out of five representative micrographs is shown in A. Bar, 20 µm. (B and C) Increased expression of PD-1 protein on the surface of tumor-infiltrating T cells from HCC patients. The samples used in B and C were fresh blood from healthy individuals and HCC patients, and paired nontumor and tumor tissues from HCC patients ( n = 7 for each). The data shown in B are representative dot plots of seven patients from six independent experiments. The horizontal bars in C represent median values. (D) HCC-derived monocytes induced anergy of tumor T cells with reduced IFN-γ production. Purified tumor T cells were left untreated (1), or were incubated for 20 h with autologous tumor monocytes supplemented with 25 µg/ml of autologous tumor mass lysate in the absence (2) or presence of 5 µg/ml of control (3) or anti–PD-L1 antibody (4). Thereafter, production of IFN-γ was determined by ELISPOT. Three out of six representative patient samples are shown in D.

Article Snippet: For immunofluorescence analysis, tissues were stained with polyclonal rabbit anti–human CD8 and mouse anti–human PD-L1, or rabbit anti–human CD68 (Santa Cruz Biotechnology, Inc.) and mouse anti–human PD-L1, followed by Alexa Fluor 488– or 568–conjugated goat anti–mouse IgG and Alexa Fluor 568– or 488–conjugated goat anti–rabbit IgG (Invitrogen).

Techniques: Derivative Assay, Expressing, Purification, Incubation, Control, Enzyme-linked Immunospot

Journal: The Journal of Experimental Medicine

Article Title: Activated monocytes in peritumoral stroma of hepatocellular carcinoma foster immune privilege and disease progression through PD-L1

doi: 10.1084/jem.20082173

Figure Lengend Snippet: TSN-exposed monocytes induced T cell suppression via PD-L1. Autologous monocytes (MO) or TSN-treated monocytes (PD-L1 + MO) were pretreated with 10 µg/ml mitomycin C for 30 min and were then washed and incubated with tumor-specific T cells (1:10) in the presence or absence of 5 µg/ml anti–PD-L1 or control antibody, as described in Materials and methods. The expression of CD25 on (A) T cells, (B) perforin in CD8 + T cells, and (C) intracellular staining of IFN-γ were determined by FACS, and (D) the secretion of cytokines and proliferation of T cells were determined by ELISA and BrdU assay, respectively. The results shown are representative of at least four separate experiments and are expressed as means ± SEM. Significant differences compared with normal monocytes are indicated (*, P < 0.05; and **, P < 0.01).

Article Snippet: For immunofluorescence analysis, tissues were stained with polyclonal rabbit anti–human CD8 and mouse anti–human PD-L1, or rabbit anti–human CD68 (Santa Cruz Biotechnology, Inc.) and mouse anti–human PD-L1, followed by Alexa Fluor 488– or 568–conjugated goat anti–mouse IgG and Alexa Fluor 568– or 488–conjugated goat anti–rabbit IgG (Invitrogen).

Techniques: Incubation, Control, Expressing, Staining, Enzyme-linked Immunosorbent Assay, BrdU Staining